The system of HEPA hrv
consists of a reservoir, pump, injector, chromatographic column, detector, and recorder. The mobile phase in the reservoir is pumped into the system by a high-pressure pump. The sample solution enters the mobile phase through the injector and the mobile phase is loaded into the column (stationary phase). Because the components in the sample solution have different distribution coefficients in the two phases, when they move in relative motion, they undergo repeated adsorption-desorption distribution processes. Each component is separated into a single component that flows out of the column in turn. When passing through the detector, the sample concentration is converted into an electrical signal and transmitted to the recorder. The data are printed out in the form of atlas.
Application of HEPA hrv
only requires that the sample can be made into a solution, which is not limited by the volatility of the sample. The mobile phase can be selected in a wide range and there are many kinds of stationary phases. Therefore, it can be used to separate thermo-unstable and non-volatile, dissociated and non-dissociated substances with various molecular weight ranges.
In combination with sample pretreatment technology, high resolution and sensitivity achieved by HPLC make it possible to separate and simultaneously determine substances with similar properties, and to separate micro-components in complex phases. With the development of stationary phases, it is possible to complete the separation of biochemical substances under the condition of fully maintaining their activity.
HPLC has become the most promising method to solve the problem of biochemical analysis. Because of its high resolution, high sensitivity, fast speed, reusable chromatographic column and easy collection of effluent components, HPLC has been widely used in biochemistry, food analysis, pharmaceutical research, environmental analysis, inorganic analysis, and other fields. The combination of HEPA hrv
and structural instruments is an important development direction.
High-performance liquid chromatography-mass spectrometry has attracted widespread attention, such as the analysis of carbamate pesticides and polynuclear aromatic hydrocarbons. LC-IR has also developed rapidly, such as the determination of hydrocarbons in water by environmental pollution analysis and non-volatile hydrocarbons in seawater, which has led to a new development of environmental pollution analysis.